Data from: Comparative chemical analysis of volatile compounds of Echinops ilicifolius using hydrodistillation and headspace solid-phase microextraction and the antibacterial activities of its essential oil

The volatile compounds from various parts of Echinops ilicifolius (Compositae) such as flowers, leaves and roots obtained by hydrodistillation (HD) and headspace solid-phase microextraction (HS-SPME) methods were subsequently analysed by GC and GC/MS and compared. Thirty-seven and 20 components of the flower of E. ilicifolius were characterized, representing 99.7% and 100.0% of the total compositions using the HD and HS-SPME methods, respectively. The major constituents of the hydrodistilled oil were identified as linalool (58.6%), geraniol (17.4%), n-dodecane (10.9%) and nerol (5.4%), whereas HS-SPME extract was rich in n-octane (34.0%), n-decane (25.1%), p-cymene (11.1%), γ-terpinene (5.3%) and 1,8-cineole (5.1%). n-Hexadecanoic acid (32.3%), linalool (16.4%) and geraniol (8.3%) were the main components among 43 constituents identified in hydrodistilled extract of the leaf, representing 99.5% of the total components detected, whereas 16 compounds representing 99.9% of the HS-SPME method were identified, among which n-decane (32.6%), p-cymene (14.0%), n-octane (10.3%), limonene (9.2%), γ-terpinene (9.1%), 1,8-cineole (7.9%) and α-pinene (5.9%) were the major ones. Among 23 components comprising 91.2% of the total hydrodistilled oil detected, n-decane (23.1%), n-dodecane (14.5%), silphiperfol-4,7(14)-diene (11.1%), selin-11-en-4-a-ol (9.5%), n-hexadecanoic acid (7.8%) and n-tetradecane (5.3%) were the main constituents in the root of E. ilicifolius, whereas 1,8-cineole (29.0%), n-decane (12.6%), n-octane (12.6%), camphor (12.0%), p-cymene (9.6%) and γ-terpinene (5.9%) were the main components among the 20 constituents characterized in HS-SPME extract, representing 99.9% of the total components detected. The antibacterial activity of the flower, leaf and root oils of E. ilicifolius against six Gram-positive and Gram-negative bacteria was determined using the MIC method. The growth inhibitory zone (mm) was also measured.

本研究采用水蒸馏法（hydrodistillation, HD）与顶空固相微萃取法（headspace solid-phase microextraction, HS-SPME），提取刺叶蓝刺头（Echinops ilicifolius，菊科Compositae）花、叶及根中的挥发性成分，随后通过气相色谱（gas chromatography, GC）与气相色谱-质谱联用法（gas chromatography-mass spectrometry, GC/MS）进行分析并对比。采用HD法与HS-SPME法分别从该植物的花中鉴定出37种与20种成分，分别占总成分的99.7%与100.0%。水蒸馏法提取的精油主要成分为芳樟醇（linalool，58.6%）、香叶醇（geraniol，17.4%）、正十二烷（n-dodecane，10.9%）与橙花醇（nerol，5.4%）；而HS-SPME提取物的主要成分为正辛烷（n-octane，34.0%）、正癸烷（n-decane，25.1%）、对伞花烃（p-cymene，11.1%）、γ-松油烯（γ-terpinene，5.3%）与1,8-桉叶素（1,8-cineole，5.1%）。从该植物叶片的水蒸馏提取物中鉴定出43种成分，其中主要成分为十六烷酸（n-hexadecanoic acid，32.3%）、芳樟醇（16.4%）与香叶醇（8.3%），占总检测成分的99.5%；而采用HS-SPME法鉴定出16种成分，占总成分的99.9%，主要成分为正癸烷（32.6%）、对伞花烃（14.0%）、正辛烷（10.3%）、柠檬烯（limonene，9.2%）、γ-松油烯（9.1%）、1,8-桉叶素（7.9%）与α-蒎烯（α-pinene，5.9%）。在该植物根的水蒸馏精油中，共检测到23种成分，占总成分的91.2%，主要成分为正癸烷（23.1%）、正十二烷（14.5%）、西柏-4,7(14)-二烯（silphiperfol-4,7(14)-diene，11.1%）、瑟林-11-烯-4-α-醇（selin-11-en-4-α-ol，9.5%）、十六烷酸（7.8%）与正十四烷（n-tetradecane，5.3%）；而采用HS-SPME法鉴定出20种成分，占总检测成分的99.9%，主要成分为1,8-桉叶素（29.0%）、正癸烷（12.6%）、正辛烷（12.6%）、樟脑（camphor，12.0%）、对伞花烃（9.6%）与γ-松油烯（5.9%）。本研究采用最低抑菌浓度（minimum inhibitory concentration, MIC）法测定了刺叶蓝刺头的花、叶与根精油对6种革兰氏阳性菌与革兰氏阴性菌的抗菌活性，并测定了其生长抑制圈直径（mm）。