Data regarding anti-quorum sensing and antimicrobial activity of Melaleuca alternifolia and Salvia sclarea essential oil against Pseudomonas aeruginosa

Researchers are now looking for alternatives that reduce the virulence and pathogenic potential of the bacteria without killing it. It can be accomplished by interfering with the quorum sensing (QS) system of bacteria. In this article, we aim to determine the antimicrobial and anti-QS activity of Salvia sclarea and Melaleuca alternifolia essential oils. The sub-lethal concentration of these essential oils (EOs) was found with the help of a growth curve, and further experiments were carried out below this concentration. To check for their anti-quorum activity, a bioreporter strain E. coli pJN105LpSC11 (to measure the concentration of 3-oxo-C12-HSL) and Chromobacterium violaceum CV026 (to check for the reduction in the formation of violacein pigment) was used. Several virulence phenotype assays like pyocyanin, alginate, and protease production, along with swarming motility, were done. The effect of these essential oils on biofilm formation was also checked. The results were confirmed by checking the expression of genes by real-time PCR.

当前研究人员正致力于探寻无需杀伤细菌，即可降低其毒力与致病潜能的替代方案。该目标可通过干扰细菌的群体感应（quorum sensing, QS）系统实现。本研究旨在探究快乐鼠尾草（Salvia sclarea）与互叶白千层（Melaleuca alternifolia）精油的抗菌活性及抗群体感应活性。研究人员通过生长曲线法确定了两种精油（essential oils, EOs）的亚致死浓度，并在此浓度以下开展后续实验。为验证其抗群体感应活性，本研究采用了两类生物报告菌株：大肠杆菌（E. coli）pJN105LpSC11，用于检测3-氧代-C12-高丝氨酸内酯（3-oxo-C12-HSL）的浓度；以及紫色色杆菌（Chromobacterium violaceum）CV026，用于检测紫菌素（violacein）生成量的变化。本研究还开展了多项毒力表型检测实验，包括绿脓菌素（pyocyanin）、藻酸盐（alginate）与蛋白酶生成量测定，以及群集运动（swarming motility）分析，并评估了两种精油对生物膜（biofilm）形成的影响。最后通过实时荧光定量PCR（real-time PCR）检测基因表达水平，验证了所有实验结果。