Campylobacter hepaticus transcriptomics revealed the genes involved in the Spotty Liver Disease (SLD) pathogenesis

Campylobacter hepaticus, the causative agent of spotty liver disease (SLD) in commercial layer hens, leads to liver necrosis, reduced egg production, and increased mortality. Despite its impact, the pathogen's virulence mechanisms remain poorly understood. This study used high-throughput transcriptomics to analyze gene expression profiles of C. hepaticus cultured in vitro bile, infected with LMH cells, and SLD affected chicken livers (in vivo) obtained from field cases to uncover key factors involved in host adaptation and pathogenicity Overall design: To investigate the differential gene expression profiles of C. hepaticus in three distinct conditions (bile, LMH cell line, and SLD infected- liver)