Additional file 1 of Transcriptomics of cumulus cells – a window into oocyte maturation in humans

Additional file 1 : Supplemental Table S1: List of primers and probes used for qPCR validation of RNAseq results. Supplemental Table S2: RNAseq quality control metrics for each cohort presented as mean ± SEM (Range). Supplemental Table S3: All differentially expressed genes between MII-CC and GV-CC cohorts. Supplemental Table S4: Differentially expressed genes known to be regulated by LH, FSH, or both with their associated fold change, and number of gene sets that are found in following leading edge analysis. Supplemental Table S5: Previously identified genes implicated in oocyte maturation in vitro that were not differentially expressed in this study. Supplemental Table S6: Top 50 genes identified by leading edge analysis ranked by the number of unique gene-sets the gene was identified in. Supplemental Table S7: Normalized read counts of all samples.

附加文件1：
补充表S1：用于RNA测序（RNAseq）结果实时定量聚合酶链式反应（qPCR）验证的引物与探针列表。
补充表S2：各队列的RNAseq质量控制指标，以均值±标准误（SEM）及对应范围呈现。
补充表S3：MII-CC队列与GV-CC队列之间的全部差异表达基因。
补充表S4：已知受促黄体生成素（LH）、促卵泡生成素（FSH）或二者共同调控的差异表达基因，及其对应的倍数变化，以及该基因在后续前沿基因集分析（leading edge analysis）中被检测到的基因集数目。
补充表S5：本研究中未发生差异表达的、既往已报道的与卵母细胞体外成熟相关的基因。
补充表S6：通过前沿基因集分析（leading edge analysis）鉴定得到的前50个基因，按每个基因所涉及的独特基因集数量进行排序。
补充表S7：所有样本的标准化读段计数。