Transcription profiling by high throughput sequencing of spleens of GPR34 knockout and wild type mice

Naive spleens as well as naive and LPS-treated dendritic cells from wildtype and GPR34-/- mice were sequenced to integrate expression profiles with protein interaction networks and find functional modules that are affected by GPR34 Expression profiles of dendritic cells and whole spleens were generated using Illumina HiSeq 2500/ Illumina HiScan本研究对野生型（wildtype）及GPR34基因敲除（GPR34-/-）小鼠的未致敏脾脏，以及未致敏和脂多糖（LPS）处理的树突状细胞（dendritic cells）进行测序，旨在将表达谱与蛋白质相互作用网络（protein interaction networks）进行整合，筛选出受GPR34调控的功能模块。本研究中树突状细胞与全脾脏的表达谱均通过Illumina HiSeq 2500/Illumina HiScan平台生成。