Association of HOX with the antibacterial activity of P. subcostata.

(A) CBB staining image of the sample after SDS–PAGE. The bands indicated by arrows were subsequently subjected to N-terminal Edman degradation-peptide sequencing analysis. (B) Alignment of the N-terminal amino acid sequence of the 29 kDa polypeptide with that of C. crispus HOX. The amino acid sequence of the 29 kDa polypeptide shared 50% homology and 95.5% similarity with that of C. crispus HOX. (C) In-gel HOX activity assay. The sample was subjected to native PAGE and then stained with 4-chloro-1-naphthol to detect HOX activity. The arrows indicate the detected bands.