Identification of LC3B's binding mRNAs through a n-RIP-seq

To identify RNAs that bind to LC3B, we employed n-RIP-seq. After obtaining the nRIP-seq data and applying a rigorous threshold for peak calling, we detected 5,285 significant peaks associated with LC3B. The coverage analysis revealed a mean coverage of 99% for LC3B-associated peaks, suggesting widespread binding throughout the transcriptome U2OS cells were subjected in to n-RIP seq sing a LC3 Ab. Ig-G was used as negative control.