Costa et al. Multiple mating rescues offspring sex ratio but not productivity in a haplodiploid exposed to developmental heat stress

Summary:
The data and scripts presented were used to assess how a sublethal high temperature (36°C) affects the reproductive system of a haplodiploid species. Using spider mites (Tetranychus urticae) developed either at 25°C or 36°C, we paired individuals in four treatments (♀25°C x ♂25°C, ♀25°C x ♂36°C, ♀36°C x ♂25°C, ♀36°C x ♂36°C), to measure the effect of temperature on the fertility of both sexes, mating and remating eagerness, and the paternity of the offspring of females with different mating histories.

Files available:
We provide 3 .R files (Script_statistical_analysis_Fertility_and_Paternity_Share, Script_statistical_analysis_Mating_Behavior, Script_figures) and 6 .csv files (Dataset_Fertility_and_Paternity_share, Dataset_altered_Fertility_and_Paternity_share, Dataset_means_Fertility_and_Paternity_share, Dataset_Mating_behavior, Dataset_means1_Mating_behavior, Dataset_means2_Mating_behavior).

Script_statistical_analysis_Fertility_and_Paternity_Share
This file contains the script used to make the statistical analysis for the Fertility and Paternity Share experiment.

Script_statistical_analysis_Mating_Behavior
This file contains the script used to make the statistical analysis for the Mating Behavior experiment.

Script_figures
This file contains the script used to create all the figures.

Dataset_Fertility_and_Paternity_share
This file contains the raw data of the effect of temperature on fertility and paternity share.

Table headers are explained below:

Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).

Temp_fem - developmental temperature of the female (25°C or 36°C).

Temp_male - developmental temperature of the male (25°C or 36°C).

Type_M - mating history of the female (SM, if single mated; DM, if double mated; MM, if multiple mated).

Rep - replicate number per treatment.

Block - day in which matings were observed.

Rtemp - temperature of the observation room.

Rhum - humidity of the observation room.

Fecundity - number of eggs laid during 4 days per female.

Unhatched_eggs - number of eggs that did not develop.

Total_daughters - number of adult daughters counted.

Total_sons - number of adult sons counted.

Total_contribution_males - sum of adult daughters and unhatched eggs.

Total_offspring - sum of adult daughters, adult sons and unhatched eggs.

Prop_daughters_ue - proportion of adult daughters in the fertilized offspring (Total_daughters / Total_contribution_males).

Prop_fert_off - proportion of adult daughters and unhatched eggs in the offspring (Total_contribution_males / Total_offspring).

Observations - any observations made in each replicate.

Dataset_altered_Fertility_and_Paternity_share
This file contains the same raw data as Dataset_Fertility_and_Paternity_share, and includes 4 more lines (in SM from each treatment) to deal with the lack of variance in Total_daughters and Unhatched_eggs, which impeded the statistical analysis.

Table headers are similar to the ones in Dataset_Fertility_and_Paternity_share.

Dataset_means_Fertility_and_Paternity_share
This file contains the means of each variable studied for the effect of temperature on fertility and paternity share.

Table headers are explained below:

Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).

Temp_fem - developmental temperature of the female (25°C or 36°C).

Temp_male - developmental temperature of the first male (25°C or 36°C).

Type_M - mating history of the female (SM, if single mated; DM, if double mated; MM, if multiple mated).

Mean_prop_fem_ue - mean proportion of daughters and unhatched eggs in the offspring per treatment and female mating history.

Mean_total_off - mean number of offspring (daughters, sons and unhatched eggs) per treatment and female mating history.

Mean_prop_fem - mean proportion of adult daughters in the fertilized offspring (daughters and unhatched eggs) per treatment and female mating history.

Dataset_Mating_behavior
This file contains the raw data of the effect of temperature on mating behavior.

Table headers are explained below:

Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).

Temp_fem - developmental temperature of the female (25°C or 36°C).

Temp_male - developmental temperature of the male (25°C or 36°C).

Type_M - mating history of the female (SM, if single mated; MM, if multiple mated).

Rep - replicate number per treatment.

Block - day in which matings were observed.

Rtemp - temperature of the observation room.

Rhum - humidity of the observation room.

Mating_rate - number of times the female mated.

Lat_sec1 - latency to the first copulation of the female.

CopYN - if the female mated a first time (1=yes, 0=no).

Cop_sec1 - duration of the first copulation of the female.

Lat_sec2 - latency to the second copulation of the female.

Cop2YN - if the female mated a second time (1=yes, 0=no).

Cop_sec2 - duration of the second copulation of the female.

Fecundity - number of eggs laid during 4 days per female.

Total_daughters - number of adult daughters counted.

Total_sons - number of adult sons counted.

Sex_ratio - proportion of adult daughters in the offspring (adult daughters and sons).

Total_adults - sum of adult daughters and sons.

Observations - any observations made in each replicate.

Dataset_means1_Mating_behavior
This file contains the means of each variable studied for the effect of temperature on mating behavior.

Table headers are explained below:

Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).

Temp_fem - developmental temperature of the female (25°C or 36°C).

Temp_male - developmental temperature of the first male (25°C or 36°C).

Mean_lat1 - mean latency to the first copulation of the female per treatment.

Mean_cop1 - mean duration of the first copulation of the female per treatment.

Mean_lat2 - mean latency to the second copulation of the female per treatment.

Mean_cop2 - mean duration of the second copulation of the female per treatment.

Dataset_means2_Mating_behavior
This file contains the means of each variable studied for the effect of temperature on mating behavior present in the supplementary material.

Table headers are explained below:

Temp_fxm_1M - developmental temperatures of the female and male paired (1st mating of the female).

Temp_fem - developmental temperature of the female (25°C or 36°C).

Temp_male - developmental temperature of the first male (25°C or 36°C).

Type_M - mating history of the female (SM, if single mated; MM, if multiple mated).

Mean_off - mean number of offspring (adult daughters and sons) per treatment and female mating history.

Mean_sexratio - mean proportion of adult daughters in the offspring (adult daughters and sons) per treatment and female mating history.