A global transcriptome analysis of human dermal fibroblasts upon knockdown or overexpression of the circular RNAs circGLIS3 (circBase ID: hsa_circ_0002874). A global transcriptome analysis of human dermal fibroblasts upon knockdown or overexpression of the circular RNAs circGLIS3 (circBase ID: hsa_circ_0002874)

In an RNAseq analysis, we have identified circGLIS3 with high levels acute wound dermis compared to skin. The biological function of circGLIS3 in human dermal fibroblasts during wound repair has not been studied. To study the genes regulated by circGLIS3, we transfected siRNA or plasmid into human dermal fibroblasts to knockdown or overexpress circGLIS3. We performed a global transcriptome analysis of fibroblasts upon circRNA knockdown or overexpression using Affymetrix arrays. Overall design: Gene expression profiling of fibroblasts transfected with siRNA control (n = 3), si-circGLIS3 (n = 3), p-Ctrl (n = 2), p-circGLIS3 (n = 2) or p-circGLIS3_RNA (n = 2) for 24 hours was carried out by using human Clariom™ S assay (ThermoFisher Scientific) at the Bioinformatics and Expression Analysis (BEA) core facility at Karolinska Institutet.