Genetic reporter for live tracing fluid flow forces during cell fate segregation in mouse blastocyst development
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https://www.ncbi.nlm.nih.gov/sra/SRP360912
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We used a robut low input cell and high throughput in-situ ChIP method to emphasize regulation mechanism among E3.5 klf2, E3.5 klf4 and E12.5 EC klf2. Overall design: We fused Protein A to the N-terminal of Tn5 to generated Protein A-Tn5 fusion enzyme, the ProteinA-Tn5-complex is enriched through specific antibodies to genomic regions and Tn5 generates indexed chromatin fragments ready for the library preparation and sequencing
创建时间：
2023-10-31
