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Parameters included in systematic evaluation of staining conditions for CD31, CD8 and FOXP3 in osteosarcoma samples.

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Systematic evaluation of more than 200 different antigen retrieval conditions, different blocking conditions, detection systems and chromogens allowed identification of optimal staining conditions for formalin-fixed and paraffin-embedded osteosarcoma samples. * In steps of 3–5°C; ** in steps of 5 min; *** in concentrations of 0.1%–10%, adjusted temperatures and various times and combinations.

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2014-03-03
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