Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells. A study of Loconte et al.

Understanding the mechanisms of viral diseases calls for techniques to analyze three-dimensional cell architecture at high resolution and speed. Rapid imaging with soft X-ray tomography visualizes SARS-CoV-2 induced cellular remodeling at the whole cell level, opening avenues to analyze in statistically significant numbers virus-cell interactions and efficacy of antiviral drugs. These datasets are reconstructed 3D volumes obtained by soft X-ray tomography on HEK293T-ACE2 cells, accompanying publication "Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells" of Loconte et al. in Cell Reports Methods. The data is organized in folders depending on the time after infection, each SXT dataset is individual *.mrc file.

解析病毒性疾病的致病机制，亟需可实现高分辨率、高速度分析细胞三维结构的技术手段。采用软X射线断层扫描（soft X-ray tomography）开展快速成像，可在全细胞层面可视化新型冠状病毒（SARS-CoV-2）诱导的细胞重构，为开展具有统计学显著性规模的病毒-细胞相互作用分析以及抗病毒药物疗效评估开辟了新路径。本数据集为基于HEK293T-ACE2细胞的软X射线断层扫描成像重构所得三维容积数据，配套Loconte等人发表于《Cell Reports Methods》期刊的论文"Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells"。数据按照感染后的时间节点分文件夹存储，每份SXT数据集均为独立的*.mrc文件。