Genetic diversity and virulence properties of caprine Trueperella pyogenes isolates

Trueperella pyogenes is an opportunistic pathogen that causes suppurative infections in various animal species, including goats. So far, only limited knowledge of phenotypic and genotypic properties of T. pyogenes isolates from goats has been gathered. The aim of this study was to assess the presence of virulence factors and biofilm formation capability, as well as the genetic diversity of caprine T. pyogenes isolates. A total of 51 T. pyogenes isolates were subjected to detailed phenotypic and genotypic characterization. The presence of eight genes encoding putative virulence factors (plo, nanH, nanP, cbpA, fimA, fimC, fimE, fimG) and one gene regulating the biofilm formation (luxS) of T. pyogenes was investigated by PCR. The assessment of the relationship of caprine T. pyogenes isolates was performed by Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) with the M13 primer (5’-GAGGGTGGCGGTTCT-3’). Data deposited in the repository was saved in .txt, .xls and .tiff format.