SIMULTANEOUS LIPID AND CAROTENOID PRODUCTION BY STEPWISE FED-BATCH CULTIVATION OF Rhodotorula mucilaginosa WITH CRUDE GLYCEROL

Abstract In the present study, an innovative cultivation strategy of Rhodotorula mucilaginosa CCT 7688 to accumulate both lipids and high added value carotenoids was proposed, with the potential to contribute to the economic feasibility of microbial oils in comparison with vegetable oils. The stepwise fed-batch cultivation with crude glycerol feeding at 24 h, 96 h, and 144 h, with the addition of magnesium to the initial culture medium, resulted in a lipid content of 51.0 ± 0.3 % CDW, biomass concentration of 21.00 ± 0.48 g L-1, total lipid production of 10.72 ± 0.18 g L-1, lipid productivity of 0.037 ± 0.001 g L-1 h-1, volumetric carotenoid production of 2843.2 ± 282.0 μg L-1, and carotenoid productivity of 9.87 ± 0.98 μg L-1 h-1. These values represent a 6-fold increase in lipid content, a 19-fold increase in lipid production, and a 2-fold increase in carotenoid production compared to batch cultivation.

摘要 本研究提出了一种创新的黏红酵母（Rhodotorula mucilaginosa）CCT 7688培养策略，可同步积累油脂与高附加值类胡萝卜素，该策略有望相较于植物油提升微生物油脂的经济可行性。本研究采用分步补料分批培养工艺，分别于24 h、96 h及144 h补加粗甘油，并在初始培养基中添加镁盐，最终获得如下结果：脂质含量达51.0 ± 0.3 % 细胞干重（Cell Dry Weight, CDW），生物量浓度为21.00 ± 0.48 g·L⁻¹，总油脂产量为10.72 ± 0.18 g·L⁻¹，油脂产率为0.037 ± 0.001 g·L⁻¹·h⁻¹，体积类胡萝卜素产量为2843.2 ± 282.0 μg·L⁻¹，类胡萝卜素产率为9.87 ± 0.98 μg·L⁻¹·h⁻¹。与分批培养相比，该工艺使脂质含量提升6倍、油脂产量提升19倍、类胡萝卜素产量提升2倍。