Sequencing of different endometrial samples

Endometrial complex atypical hyperplasia (CAH) is the precancerous lesion of endometrial cancer. However, the underlying mechanism by which it progresses to endometrial cancer remains elusive. Here, we performed RNA sequencing on endometrial cancer, CAH, reversed CAH and normal endometrium samples to reveal key genes implicated in this transition. CEBPB was found to paricipate in CAH and promotes its progression. Using primary cultures of CAH, which were also subjected to RNA sequencing after knowdown of CEBPB using siRNA, we further confimed the biological effects of CEBPB in CAH.