Microarray expression profiling of mRNAs in the developing murine first molar tooth germs

The global gene expression of murine tooth germs dissected at various stages of tooth development was investigated. A list of genes differentialy expressed throughout the various time points was submitted to bioinformatic analysis using Ingenuity Pathway Analysis (IPA) to identify significant associations between clusters of genes found to be differentially expressed and signalling or canonical pathways or molecular/cellular functions. This approach was also used with clusters of genes isolated using the profile search function of Spotfire.). The study entailed a total of 58 dual-labeled/two-channel microarrays.The results, encompassing at least triplicate microarray data at each time-point, were treated as single colored arrays. The resulting expression data was subsequently assembled into a single data file, facilitating analysis of the time-course of expression profiles of expressed genes.本研究针对不同牙齿发育阶段分离的小鼠牙胚的全局基因表达谱展开了探究。针对各时间点下差异表达的基因列表，采用Ingenuity通路分析（Ingenuity Pathway Analysis, IPA）进行生物信息学分析，以鉴定差异表达基因簇与信号通路、经典通路或分子/细胞功能之间的显著关联。该分析策略同样被应用于通过Spotfire的剖面搜索功能分离得到的基因簇。本研究共包含58张双标记/双通道微阵列芯片。各时间点的微阵列数据均包含至少三次生物学重复，原始数据被按单通道阵列进行处理。最终得到的表达数据被整合为单个数据文件，以便对表达基因的表达谱时间进程开展分析。