Ca2+/calmodulin-dependent protein kinase II tetracycline-transactivator expression: coronal sections (case 317.8)

Bright-field microscopy images of serial coronal brain sections showing Ca2+/calmodulin-dependent protein kinase II (Camk2a) promoter expression in a bigenic Camk2a-tetracycline-transactivator (tTA) mouse brain (case 317.8, adult female), using a driver-reporter construct in which the Camk2a-tTA promoter regulates the expression of the E. coli derived LacZ reporter gene encoding β-galactosidase, which is visualized histologically using X-gal (5-Bromo-4-chloro-3-indolyl β-d-galactopyranoside) as a substrate. Methodological details are provided in Odeh et al., Neuroimage 54:2603-11, 2011. Our data show that the Camk2a-tTA promoter is spatially expressed in multiple brain regions, including the cerebral cortex, several basal ganglia regions, hippocampus, tectum, and cerebellum.