The effect of G-quadruplex on gene expression by treating S2 cell with different concentration of TMPyP4

In this study, G-quadruplex ligand TMPyP4 was utilized to treat S2 cell and DMSO was served as control. The stabilization role of TMPyP4 will promote the formation of G-quadruplex and then affect gene expression. Identifying whether the effect is positive or negative is important for our understanding of the functionality of non-coding element. Overall design: We treated S2 cell with different concentration of TMPyP4 (25µmol/L, 50µmol/L, 100µmol/L). We designed three experimental groups and one control group. The experimental group 1 was treated with 25µmol/L alone, the experimental group 2 was treated with 50µmol/L alone, and the experimental group 3 was treated with 100µmol/L alone. The drug solvent DMSO-treated group served as a control group, with 3 replicates per treatment in each group.