Summary of real-time PCR data generated from Colletotrichum cereale samples using Apn2 detection assay for clade A and clade B.

700 samples were evaluated, plus ten additional non-target Colletotrichum and other species included as negative controls. Second derivative CT (cycle threshold) values represent positive samples when the fluorescent signal crosses the amplification threshold prior to cycle 40. Average CT values are based on a minimum of three technical replicates.aThe 35 biological replicates are not included in the count of positive reactions.bAverage CT values are given as the mean CT generated from all technical and biological replicates.cLow fluorescence intensity and late CT values (>40.0) were observed for these samples when tested using the B-Apn2 assay.