Arbuscular mycorrhizal communities respond to nutrient enrichment and plant invasion in phosphorus-limited eucalypt woodlands

Arbuscular mycorrhizal fungi (AMF) facilitate ecosystem functioning through provision of plant hosts with phosphorus (P), especially where soil P is limiting. Changes in soil nutrient regimes are expected to impact AMF, but the direction of the impact may depend on context. We predicted that nitrogen (N)-only enrichment promotes plant invasions and exacerbates their P limitation, increasing the utility of AMF and promoting AMF diversity. We expected that enrichment with N, P and other nutrients similarly promotes plant invasions, but decreases the benefit and diversity of AMF because P is readily available for both native and exotic plants. We tested these hypotheses in eucalypt woodlands of south-western Australia, that occur on soils naturally low in P. We evaluated AMF communities within three modified ground-layer states representing different types of nutrient enrichment and associated plant invasions. We compared these modified states to near-natural reference woodlands. AMF richn..., Site selection
Sampling was centred on two nature reserves with areas of York gum (Eucalyptus loxophleba subsp. loxophleba) – Jam (Acacia acuminata) woodlands (hereafter York gum woodlands) in close to reference condition. The reserves are located in the central wheatbelt, Western Australia: Mount Caroline (31°45'25.3\"S, 117°38'38.3\"E) and Namelkatchem Nature Reserves (31°10’47.9”S, 117°11’18.1”E), and are ~ 70 km apart (Fig. 1). These reserves have a history of minimal livestock grazing and plant invasion, permitting the persistence of large areas of diverse understoreys dominated by native perennial and annual forbs and grasses (Prober and Wiehl 2012). The climate is Mediterranean type, with long-term (1990–2022) mean annual temperature and rainfall, respectively, of 17.8 °C and 321 mm at Mt Caroline and 17.6 °C and 333 mm at Namelkatchem (BoM, 2023).
Our experimental design involved four ground-layer states representing different levels of nutrient enrichment and plant invasions as d..., , # Arbuscular mycorrhizal communities respond to nutrient enrichment and plant invasion in phosphorus-limited eucalypt woodlands

[https://doi.org/10.5061/dryad.c866t1gfw](https://doi.org/10.5061/dryad.c866t1gfw)

This data was used to conduct all analyses of the linked article. It contains treatments allocated to each sample, as well as all soil chemistry, vegetation, and arbuscular mycorrhizal fungi (AMF) variables

## Description of the data and file structure

Three data files are provided: a raw community matrix of AMF (i.e. prior rarefaction), a raw community matrix of plants, and a data frame with the other is the environmental data frame with all soil chemistry, vegetation cover and richness, and AMF richness. Please refer to the published article for methodology on how these data were collected.

All three data files are connected by the \"sample\" column from each data file.

Each data file is in excel form and has two sheets: one with the data, and one with the metadata that exp...

丛枝菌根真菌（Arbuscular mycorrhizal fungi, AMF）可通过为宿主植物供给磷（phosphorus, P）助力生态系统功能实现，尤其在土壤磷素匮乏的生境中。土壤养分状况的变化被认为会对丛枝菌根真菌产生影响，但影响方向可能取决于具体情境。我们提出如下假说：仅添加氮（nitrogen, N）的养分富集会促进植物入侵，加剧植物的磷素限制胁迫，从而提升丛枝菌根真菌的功能效用并推动其群落多样性提升；而同时添加氮、磷及其他养分的富集同样会促进植物入侵，但会降低丛枝菌根真菌的收益与群落多样性，因为此时本土与外来植物均可直接获取磷素。我们在澳大利亚西南部的桉树林中验证了上述假说，该区域土壤天然磷素含量较低。我们对三种代表不同养分富集类型与关联植物入侵的改造地表层生境状态下的丛枝菌根真菌群落进行了评估，并将这些改造样地与近自然参照林分进行了对比。丛枝菌根真菌丰富度……

## 样地选择
采样工作聚焦于两处接近参照状态的自然保护区，其境内分布有约克胶木（Eucalyptus loxophleba subsp. loxophleba）- 细叶相思（Acacia acuminata）林（下文简称约克胶木林）。两处保护区均位于澳大利亚西部中部小麦种植带：卡罗琳山（31°45'25.3"S, 117°38'38.3"E）与纳梅尔卡特切姆自然保护区（31°10’47.9"S, 117°11’18.1"E），两者间距约70公里（图1）。这两处保护区的历史放牧与植物入侵程度极低，因此保留了大面积以本土多年生、一年生杂类草与禾草为优势种的多样林下植被（Prober与Wiehl, 2012）。两地气候均属地中海型，卡罗琳山站1990-2022年的年均温为17.8℃，年降水量321mm；纳梅尔卡特切姆站同期年均温17.6℃，年降水量333mm（澳大利亚气象局（Bureau of Meteorology, BoM）, 2023）。

我们的实验设计包含四种代表不同养分富集水平与植物入侵程度的地表层生境状态，以……

# 磷限制桉树林中丛枝菌根群落对养分富集与植物入侵的响应
https://doi.org/10.5061/dryad.c866t1gfw

本数据集用于支撑关联论文的全部分析内容，包含每个样本对应的处理组信息，以及所有土壤化学、植被与丛枝菌根真菌（AMF）相关变量。

## 数据与文件结构说明
本次提供三份数据文件：一份为丛枝菌根真菌的原始群落矩阵（即稀疏化（rarefaction）前的群落数据），一份为植物原始群落矩阵，另一份为环境数据框，包含所有土壤化学指标、植被盖度与物种丰富度以及丛枝菌根真菌丰富度数据。
三份数据文件均通过各文件中的"sample"列进行关联。
每份数据文件均为Excel格式，包含两个工作表：一个工作表存储数据内容，另一个工作表存储元数据（metadata）以详述……