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Leucine effect on miR-494-3p and miR-696 and mitochondrial biogenesis in C2C12 myotubes

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doi.org2025-03-26 收录
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http://doi.org/10.17632/sd7dxkc7r6.2
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资源简介:
- Western Blot images, semi-dry Samples: C2C12 myotubes treated with Control (H2O), Leu 1mM, Leu 3mM or Caff 3mM for 24h, applied in that order. - Raw data for statistics (Excel file). - Raw RT-qPCR data for the detection of miRNA expression levels and mitochondrial DNA copy numbers. Measurements were made by duplicates and relative expression were calculated using the ΔΔCt method.

西氏印迹图像,半干式处理。样本包括经对照组(H2O)处理的C2C12肌管细胞、经1mM亮氨酸处理的Leu、经3mM亮氨酸处理的Leu或经3mM咖啡因处理的Caff处理24小时后的样本,处理顺序如上所述。统计数据原始数据(Excel文件)。检测miRNA表达水平和线粒体DNA拷贝数的原始RT-qPCR数据。测量结果以重复次数计算,相对表达量通过ΔΔCt方法计算得出。
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