Global gene expression profile of colon from wild-type and AE2-knockout mice

The objective of this study was to identify changes in gene expression levels between wild-type and AE2-knockout colon. 7 wild-type and 7 AE2-null colon RNA samples were compared to identify genes that are differentially expressed in the colon of AE2 knockout mice. Total RNA was collected from both male and female wild-type and AE2-null colons (including both proximal and distal colon) when the mice were 18 days old. All pairs were gender- and aged-matched, and all mice were on a mixed 129 Black Swiss background. To facilitate statistical analysis and reduce any affects of Cy3 and Cy5 labeling, comparisons of two wild-type and two AE2-null samples were repeated using a dye flip.本研究旨在探究野生型（wild-type）与AE2基因敲除（AE2-knockout）结肠组织间的基因表达水平变化。本研究纳入7份野生型结肠RNA样本与7份AE2基因缺失（AE2-null）结肠RNA样本，通过比对筛选出AE2敲除小鼠结肠组织中差异表达的基因。实验采集了18日龄小鼠的野生型与AE2基因缺失结肠（包含近端结肠与远端结肠）组织的总RNA，样本涵盖雄性与雌性个体。所有样本均进行了性别与年龄匹配，且所有实验小鼠均为129 Black Swiss混合遗传背景。为便于统计学分析并降低Cy3与Cy5荧光标记带来的系统误差，本研究对2份野生型样本与2份AE2基因缺失样本采用染料翻转（dye flip）策略进行了重复比对实验。