Responses of Antarctic marine biota to contaminants - Brown Ostracod toxicity tests, Kingston 2007

Metadata record for data from AAS (ASAC) Project 2933.While it is generally thought that Antarctic organisms are highly sensitive to pollution, there is little data to support or disprove this. Such data is essential if realistic environmental guidelines, which take into account unique physical, biological and chemical characteristics of the Antarctic environment, are to be developed. Factors that modify bioavailability, and the effects of common contaminants on a range of Antarctic organisms from micro-algae to macro-invertebrates will be examined. Risk assessment techniques developed will provide the scientific basis for prioritising contaminated site remediation activities in marine environments, and will contribute to the development of guidelines specific to Antarctica.Brown Ostracod toxicity tests, Kingston 2007Test animals were collected from near shore environments at Casey Station, East Antarctica during Dec 2006 - Jan 2007, and transported to culturing facilities at the Australian Antarctic Division in Tasmania, where tests were conducted during 2007. The test animals were exposed to metals in non-renewable static tests in vials containing 50 mL of the test solution at ambient Antarctic coastal salinity of 34 ppt. Tests were held in temperature controlled cabinets (incubators) at a temperature of 0, 2 or 4 degrees C (+/- approximately 1 degrees C). Ten test animals were introduced into each of 3 to 5 replicate vials per treatment at test commencement, and were exposed for 10 weeks during which periodic observations were made. Test solutions were renewed in weekly water changes.Periodic observations (time since start of test) are given in hr (hours), d (days) or w (weeks). At each observation time, test animals were scored in one of the Endpoint categories described on each worksheet. Each worksheet provides data for a particular test taxa (Brown Ostracods - taxonomy to be verified) for a given test number (T01, T02, T03, T04, T05,) and a given metal contaminant (copper, zinc, cadmium and lead).Test information is provided in the first 14 rows of each worksheet, e.g. Site of collection, Test start date, Endpoint categories etc. ASU = artificial settlement units (plastic scourers used by Sarah Richards, which had been deployed in Newcomb Bay in approximately the year 2000).Concentration micro grams per litre are nominal concentrations. Measured concentrations are provided in the file: Brown Ostracod_chemistry.xls, as described below. Test temperature was 0 degrees C unless otherwise stated. Unit for all temperature data are degrees C. See the readme file in the download for more information.

本数据集为AAS（ASAC）项目2933的元数据记录。学界普遍认为南极生物对污染高度敏感，但目前鲜有相关数据可佐证或推翻这一观点。若要制定兼顾南极环境独特物理、生物与化学特征的务实环境导则，此类数据必不可少。本研究将探究影响生物有效性的各类因素，以及常见污染物对从微型藻类到大型无脊椎动物的各类南极生物的影响。所开发的风险评估技术，将为海洋环境污染场地修复优先级排序提供科学依据，并助力制定适配南极的专属环境导则。

棕色介形类（Brown Ostracod）毒性试验（2007年，金斯顿）
试验动物于2006年12月至2007年1月采自南极东部凯西站近岸海域，随后被转运至塔斯马尼亚州澳大利亚南极分部的养殖设施中，并于2007年完成全部试验。
试验采用静态非更新式暴露体系，将10只试验动物置于含50mL试验溶液的玻璃瓶中，溶液盐度维持南极沿岸盐度标准34 ppt。试验在控温培养箱中进行，设置0℃、2℃或4℃三个温度梯度（误差约±1℃）。每个处理组设置3至5个平行样瓶，试验开始时每瓶接入10只试验动物，暴露时长为10周，期间进行定期观测。试验溶液每周通过换水更新。

定期观测的时间以试验开始后的时长计，单位可记为小时（hr）、天（d）或周（w）。在每个观测节点，试验动物将根据各工作表中定义的终点类别进行评分。每张工作表对应特定试验编号（T01、T02、T03、T04、T05等）、特定金属污染物（铜、锌、镉、铅）以及特定试验类群（棕色介形类——分类学信息待验证）的相关数据。

每张工作表的前14行包含试验基本信息，例如采集地点、试验开始日期、终点类别等。ASU（artificial settlement units，人工附着单元）指莎拉·理查兹使用的塑料刷洗球，该装置于2000年左右部署在纽科姆湾。

文中所标注的浓度单位为微克每升，均为名义浓度。实测浓度详见附件文件Brown Ostracod_chemistry.xls，具体说明如下。除特别标注外，试验温度均为0℃，所有温度数据的单位均为摄氏度。更多详细信息请参阅下载包中的readme文件。