Next Generation Sequencing Analysis of miRNA profiling in Epithelial Type and Mesenchymal Type Beas-2b Cells. Next Generation Sequencing Analysis of miRNA profiling in Epithelial Type and Mesenchymal Type Beas-2b Cells

We reported the application of next generation sequencing technology for high-throughput profiling of miRNA expression in bronchile epithelial cell line Beas-2b with epithelial or mesenchymal mophology. By comparation the expression aboundence of known miRNAs between epithelial type and mesenchymal type Beas-2b cells, we found both upregulated and downregulated miRNAs in bronchial epithelial cells during EMT. This study provides a basic condition for further investigation of the roles of the regulated miRNAs during EMT in bronchial epithelial cells. Overall design: Examination of miRNA profiling in epithelial and mesenchymal subtypes of Beas-2b cells