Alterations of gut microbiota and liver mitochondrial function involved in the maternal physiological response to late gestational heat stress in a mouse model

We first observe the changes in both maternal physiological status (temperature, feed intake, water consumption) and metabolic profiles (body composition and serum metabolic parameters). Further, HS effects on the mitochondrial function and microbial structure are investigated by detecting mRNA expressions of liver mitochondrial functional genes and relative abundance of potential microbial drivers, respectively. Finally, the random forest algorithm and multiple linear regression are used to predict the contribution of potential microbial drivers for mitochondrial function and metabolic profiles.