Single-cell transcriptomics of mesenchymal stem/stromal cells from human umbilical cord

Mesenchymal stem/stromal cells (MSCs) demonstrate great therapeutic potential in regenerative medicine. However, the differences between the transcriptome of MSCs from umbilical cord tissue and in vitro cell culture at single-cell level remain elusive. We obtained a large-scale single-cell transcriptome of total 19221 single cells from human umbilical cord Wharton's jelly tissue and the matched culture-expanded MSCs from three donors to provide a high quality transcriptomic profiling dataset at single-cell resolution, which is a valuable resource for dissecting the MSCs heterogeneity, cell fate decision in native tissue niche and in vitro culture environment for specific lineages.