Accelerated aging test in niger seeds

Abstract:Niger is a promising oilseed species for biodiesel production but there is no much information about the physiological potential of its seeds. Thus, the aim was to adapt the methodologies of accelerated aging test on six lots of niger seeds. The test was carried out by traditional and with saturated salt solution (20 and 40 g NaCl.100 mL-1) methods at 41 and 45 °C for 24, 48, 72 and 96 hours. After the decay period, the seeds were submitted to the germination test, proceeding to an evaluation on the seventh day after sowing, counting the normal seedlings percentage. A completely randomized design with four replications of 50 seeds was used and the means were compared by Tukey's test. The accelerated aging test was correlated with seedling emergence and provided lots classification in at least two levels of vigor. For the accelerated aging test, the method with 20 g NaCl.100 mL-1 at 41 °C for 24 hours is recommend. The traditional method is not suitable because it provides water content variation between samples above what is tolerable.

摘要：尼日尔油籽（Niger）是一种极具应用前景的生物柴油生产油料物种，但目前关于其种子生理潜力的相关研究信息较为匮乏。为此，本研究旨在针对六批尼日尔油籽种子，优化加速老化试验（accelerated aging test）的方法体系。试验分别采用传统方法以及饱和氯化钠溶液（20g NaCl·100mL⁻¹和40g NaCl·100mL⁻¹）两种处理方式，在41℃与45℃条件下分别处理24、48、72及96小时。老化处理结束后，对种子开展发芽试验（germination test），于播种后第七天进行评估并统计正常幼苗百分率。本试验采用完全随机试验设计（completely randomized design），每组设置4次重复，每次重复包含50粒种子，组间均值比较采用Tukey检验（Tukey's test）。加速老化试验结果与幼苗出苗率（seedling emergence）显著相关，可将供试种子批划分为至少两个种子活力（seed vigor）等级。针对加速老化试验，推荐采用20g NaCl·100mL⁻¹的饱和盐溶液法，在41℃条件下处理24小时。传统方法因会导致样品间含水量波动超出可耐受范围，因此不适用于该试验。