Supplementary Figure 5, (j) of the paper Feedback regulation through myosin II confers robustness on RhoA signalling at E-cadherin junctions.

Confocal images showing the localization of E-cadherin, ZO-1 and p190B in wild type (ctrl) , N-WASP KD and Cdc42 KD cells. MCF-7 cells transfected with non-targeting control siRNA (Ctrl) or siRNA targeted against Cdc42 (Cdc42 KD) or N-WASP (N-WASP KD) were fixed with methanol and stained for p190B Rho GAP, E-cadherin or ZO-1.

共聚焦显微镜图像展示了野生型（对照组，ctrl）、N-WASP敲低（N-WASP KD）以及Cdc42敲低（Cdc42 KD）细胞中E-钙粘蛋白（E-cadherin）、ZO-1（ZO-1）和p190B的定位情况。本研究所用的MCF-7细胞分别转染了非靶向对照小干扰RNA（siRNA，Ctrl）、靶向Cdc42的siRNA（Cdc42 KD）或靶向N-WASP的siRNA（N-WASP KD），随后经甲醇固定，并针对p190B Rho GTP酶激活蛋白（p190B Rho GAP）、E-钙粘蛋白（E-cadherin）或ZO-1进行了染色。