Improved antimelanogenesis and antioxidant effects of polysaccharide from Cuscuta chinensis Lam seeds after enzymatic hydrolysis

Cuscuta chinensis polysaccharide (CPS) was extracted using hot water and enzymatically hydrolyzed C. chinensis polysaccharide (ECPS) was produced by the mannase enzymatic hydrolysis process. The purpose of this research was to investigate the antimelanogenic activity of ECPS and CPS in B16F10 melanoma cells. The in vitro antioxidant activity was assessed by their ferric iron reducing power and DPPH free radical scavenging activities. The molecular mass distribution of polysaccharides was determined using SEC-MALLS-RI. CPS was successfully enzymatically degraded using mannase and the weighted average molecular weights of CPS and ECPS were 434.6 kDa and 211.7 kDa. The results of biological activity assays suggested that the enzymatically hydrolyzed polysaccharide had superior antimelanogenic activity and antioxidant effect than the original polysaccharide. ECPS exhibited antimelanogenic activity by down-regulating the expression of tyrosinase, MITF, and TRP-1 without cytotoxic effects in B16F10 melanoma cells. In conclusion, ECPS have the potential to become a skin whitening product.

本研究采用热水提取法制备菟丝子多糖（Cuscuta chinensis polysaccharide, CPS），并通过甘露聚糖酶酶解工艺得到酶解菟丝子多糖（enzymatically hydrolyzed C. chinensis polysaccharide, ECPS）。本研究旨在探究ECPS与CPS在B16F10黑色素瘤细胞中的抗黑素生成活性。体外抗氧化活性通过铁还原能力与DPPH自由基清除能力两项指标进行评价。多糖的分子量分布采用体积排阻色谱-多角度激光光散射-示差折光检测联用系统（SEC-MALLS-RI）进行测定。本研究成功利用甘露聚糖酶对CPS进行酶解降解，CPS与ECPS的重均分子量分别为434.6 kDa与211.7 kDa。生物学活性实验结果表明，酶解多糖的抗黑素生成活性与抗氧化效果均优于原始多糖。ECPS可通过下调酪氨酸酶、小眼畸形相关转录因子（Microphthalmia-associated transcription factor, MITF）以及酪氨酸酶相关蛋白1（tyrosinase-related protein 1, TRP-1）的表达发挥抗黑素生成作用，且对B16F10黑色素瘤细胞无细胞毒性。综上，ECPS具备开发为美白护肤产品的潜力。