Enhanced Proteome Profiling by Inhibiting Proteolysis with Small Heat Shock Proteins
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https://figshare.com/articles/dataset/Enhanced_Proteome_Profiling_by_Inhibiting_Proteolysis_with_Small_Heat_Shock_Proteins/3252550
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资源简介：
Proteolytic degradation is one of the critical
problems in two-dimensional electrophoresis (2-DE). Here
we report that small heat shock proteins (sHsps), including
IbpAEc and IbpBEc from Escherichia coli and Hsp26Sc from
Saccharomyces cerevisiae, are able to protect proteins in
vitro from proteolytic degradation. Addition of sHsps
during 2-DE of human serum or whole cell extracts of E.
coli, Mannheimia succinciproducens, Arabidopsis thaliana,
and human kidney cells allowed detection of up to 50%
more protein spots than those obtainable with currently
available protease inhibitors. Therefore, the use of sHsps
during 2-DE significantly improves proteome profiling by
generally enabling the detection of many more protein
spots that could not be seen previously.
Keywords: small heat shock proteins • two-dimensional gel
electrophoresis • proteome profiling • protease inhibitor
创建时间：
2016-05-05
