RNA-seq profiling of adult skeletal muscle after long term conditional knockout of Hic1 [TA RNA-seq]

To determine the effects of long term deletion of the transcriptional repressor Hypermethylated in cancer 1 (Hic1) on skeletal muscle, samples were collected of both wild type mice and those conditionally deleted for Hic1 and profiled by RNA sequencing. Aspects of muscle homeostasis were negatively impacted, highlighting cell and non cell autonomous roles for Hic1 in maintaining functional MPs and muscle tissue.

为明确转录抑制因子癌症中高甲基化1（Hypermethylated in cancer 1, Hic1）的长期缺失对骨骼肌的影响，研究人员采集了野生型小鼠与Hic1条件性敲除小鼠的样本，并通过RNA测序（RNA sequencing）对样本进行转录组分析。实验结果显示，肌肉稳态的多项指标受到负面影响，这凸显了Hic1在维持功能性肌祖细胞（Muscle Progenitors, MPs）与肌肉组织过程中所具备的细胞自主性与非细胞自主性调控作用。