Organ-specific Sympathetic Innervation Defines Visceral Functions

We performed single-cell RNA-seq (10x Chromium 3' v.3.1) on dissociated single cell suspensions from mouse (C57BL/6) celiac and superior mesenteric sympathetic ganglia to determine transcriptomic cell types. We identified sympathetic neuron types that specifically innervate unique combinations of visceral organs and control physiological functions. We used 20 animals in total, with male and female mice in equal measure. All animals were 7-8 weeks of age.