Immediate Early Response 2 (Ier2) overexpression effect in NIH 3T3 cells. Immediate Early Response 2 (Ier2) overexpression effect in NIH 3T3 cells

Analysis of Immediate Early Response 2 (Ier2)-inducible NIH 3T3 cells after Ier2 induction with RheoSwitch ligand RSL-1. Results provide insight into the function of Ier2 in NIH 3T3 mouse embryonal fibroblasts. Immediate early genes, including Ier2, are rapidly induced in quiescent cells by proliferation and migration-inducing stimuli. Microarray gene expression profiling was performed to identify differentially expressed genes following overexpression of Ier2 in NIH 3T3-Ier2 inducible cells after 24 hour induction of Ier2. Overall design: To prepare inducible NIH 3T3-RheoSwitch-Ier2 cells, NIH 3T3 cells were transfected with pNEBR-Ier2-hygro and pNEBR-R1 plasmids (Wallbaum et al., 2009, Biotechniques, 46: 433-440; PMID: 19480637). To induce Ier2 expression, NIH 3T3-RheoSwitch-Ier2 cells were treated with RheoSwitch ligand RSL-1.