GE changes TTY23, AT22 & TAT12 vs S288C

The thermotolerant TTY23, acid tolerant AT22, and thermo-acid tolerant TAT12 yeast strains, generated by adaptive evolution experiments were grown at 30°C, pH 5.0 in the absence of acetic acid (optimal ancestral conditions). The mRNA of each strain was isolated and changes in expression were compared against the parental strain S288C.通过适应性进化实验获得的耐热TTY23、耐酸AT22以及耐热耐酸TAT12酵母菌株，在30℃、pH 5.0且无乙酸的最优祖先培养条件下进行培养。随后分离提取各菌株的信使核糖核酸（mRNA），并将其基因表达变化与亲本菌株S288C进行对比分析。