Gene expression in clavicular cortical hyperostosis

Clavicular cortical hyperostosis (CCH) is a sterile inflammatory bone disorder of unknown etiology, clinically characterized by pain and/or swelling of the clavicle. The aim of the study was to identify and confirm specific gene expression patterns in patients with CCH. Total RNA was isolated from whole blood of 18 children with diagnosis of CCH, as well as 8 age and sex matched control participants without diagnosis of chronic inflammatory disease. Of those, 5 patients and 4 controls were utilized in the microarray experiments, while the rest were used in the qPCR analysis.