Triplication of the Ifnr locus exacerbates craniofacial anomalies in a mouse model of Down syndrome.

Micro-computed tomography (mCT) reconstructions of skulls from the Dp16 mouse model of Down syndrome, Dp16 corrected for <em>Ifnr </em>dosage (Dp16^2xIfnrs), and wildtype controls (WT). Mice were decapitated at 7-8 weeks of age then whole heads imaged using a SkyScan 1275. Scanning was performed at 17.6-micron resolution using the following parameters: 55 kV, 180 mA, 0.5 mm Al filter; 0.3˚ rotation step over 180˚, and 3-frame averaging. All raw scan data were reconstructed to multiplanar slice data using NRecon v1.7.4.6 (Bruker, Belgium). Reconstructed data were then rendered in 3D with consistent thresholding parameters using Drishti v2.6.5 for gross visual assessment of the craniofacial skeleton. Representative rendered images were captured and processed using Photoshop 24.2.0 (Adobe Creative Cloud). <br>

本数据集涵盖唐氏综合征Dp16小鼠模型、经Ifnr剂量校正的Dp16小鼠（Dp16^2xIfnrs）以及野生型对照（WT）的颅骨微型计算机断层扫描（Micro-computed tomography，mCT）重建结果。实验小鼠于7-8周龄时断头取材，随后使用SkyScan 1275扫描仪对完整头部进行成像。扫描参数设置如下：分辨率17.6微米，管电压55kV，管电流180mA，配备0.5mm铝滤片；扫描角度覆盖180°，旋转步距0.3°，采用3帧平均成像模式。所有原始扫描数据均通过比利时布鲁克公司的NRecon v1.7.4.6软件重建为多平面断层切片数据。随后利用Drishti v2.6.5软件，采用统一的阈值参数对重建数据进行三维渲染，以开展颅面骨骼的大体视觉评估。代表性渲染图像通过Adobe Creative Cloud套件中的Photoshop 24.2.0软件完成采集与后期处理。