Genes with significant negative log2 ratios from DSSA identified in at least three of the six CAT treatments.

The requirement of a gene for optimal growth in CAT is quantified with a fitness score, defined as the normalized log2 ratio of the deletion strain's growth. A total of 33 (14%) of the identified genes were important for fitness (i.e. had negative fitness scores) in at least three of the six CAT treatments. Most genes identified were significant in one or two treatments and after 15 g of growth. See Table S2 for the list of all identified genes.