Molecular detection and clinical aspects of porcine circovirus 3 infection in pigs from Brazil

ABSTRACT Porcine circovirus 3 (PCV-3) DNA has been detected in serum samples from apparently healthy pigs as well as pigs with different clinical conditions. Molecular detection of PCV-3 was observed in swine serum samples from Southeastern - Brazil using a nested PCR designed specifically for this study. The epidemiology and clinical aspects of PCV-3 infection were evaluated. The samples originated from 154 pigs of both genders from different production phases and with different clinical presentations, sampled from 31 pig farms visited between 2013 and 2018. In this study, PCV-3 was detected in 26.7% of samples from all populations across varying ages. Statistical association (P=0.0285) was observed only between animals with respiratory signs and PCV-3; no PCV-3-positive animal had diarrhea. No statistical association was observed between PCV-3 and age, or gender of the pigs. Because PCV-3 is a newly discovered virus, there is very little information about its epidemiology. We hope that these data can help in future studies investigating PCV-3 epidemiology.

摘要：本研究在看似健康猪只以及出现不同临床症状的猪只的血清样本中，均检出猪圆环病毒3型（Porcine circovirus 3, PCV-3）DNA。本研究采用专为本次实验设计的巢式聚合酶链式反应（nested PCR），在巴西东南部的猪血清样本中实现了PCV-3的分子检测，并对PCV-3感染的流行病学特征与临床表型进行了评估。本次研究的样本采集自2013年至2018年间走访的31家养猪场，涵盖154头不同性别、不同养殖阶段且呈现不同临床症状的猪只。本研究中，各年龄段猪只的样本PCV-3总检出率为26.7%。统计学分析仅显示出现呼吸道症状的猪只与PCV-3感染存在显著关联（P=0.0285）；所有PCV-3检测阳性的猪只均未出现腹泻症状。未观察到PCV-3感染与猪只年龄、性别存在统计学关联。由于PCV-3是一种新近发现的病毒，目前关于其流行病学的研究资料十分有限。本研究所得数据可为未来开展PCV-3流行病学相关研究提供参考依据。