Translational profiling in undifferentiated spermatogonia

Germline-specific RiboTag IP-seq from postnatal testes synchronized for undifferentiated spermatogonia. Overall design: Germline ribosomes were specifically tagged via Ddx4Cre, which activated expression of the RiboTag allele (Rpl22HA). Spermatogenesis in postnatal testes was chemically synchronized to obtain testes enriched for undifferentiated spermatogonia. For each synchronized sample collected from a single animal, RiboTag IP-seq and input RNA-seq was carried out. Synchronized samples lacking the RiboTag allele were used as negative controls for the IP-seq. RiboTag and control samples were each generated in triplicate.