RNAseq processed data from FAT1 KOs

The gene expression data (processed files) presented here are derived from primary tongue keratinocytes (epithelial cells) isolated from WT and FAT1 knockout mice. The knockout mice were generated through Krt14-Cre mediated Fat1 deletion targeting: 1) exon 2 (Fat1tm1.1Atuf, referred to as KO-Ex2 or KO-Ex), leading to total FAT1 loss, 2) The FAT1 transmembrane domain deletion (encoded by exons 23-24) (Fat1tm1.1Fhel referred to as KOTM). Image analysis and base calling were conducted by the NovaSeq Control Software (NCS). Raw sequence data (.bcl files) generated from Illumina NovaSeq was converted into fastq files and de-multiplexed using Illumina bcl2fastq 2.19 software. Sequence reads were trimmed to remove possible adapter sequences and nucleotides with poor quality using Trimmomatic v.0.36. The trimmed reads were mapped to the Mus musculus GRCm38 reference genome available on ENSEMBL using the STAR aligner v.2.5.2b. The STAR aligner is a splice aligner that detects splice junctions and incorporates them to help align the entire read sequences. BAM files were generated as a result of this step. Unique gene hit counts were calculated by using featureCounts from the Subread package v.1.5.2. The hit counts were summarized and reported using the gene_id feature in the annotation file. Only unique reads that fell within exon regions were counted. If a strand-specific library preparation was performed, the reads were strand-specifically counted.