RNA sequencing of Patient-Derived Xenografts

Goal: Comparison of mRNA expression profiles in patient-derived xenograft primary tumors Methods: RNA sequencing performed at the High-Throughput Genomics and Bioinformatics Analysis Core at the University of Utah. Detailed procedures available in methods and DNA processing pipline sections. Results: Focus of study compared mRNA expression profiles of samples pre and post passaging in vivo. Unsupervised heirarchical clustering revealed samples clustered together appropriately. Conclusions: The PDX maintain the gene expression profiles of the original tumor. Six primary tumor samples are included in the analyses. These same six samples were sequenced again after being passed in mice. Replicates are included.