Single Cell Multiomics Profiling for the Mouse Polycystic Kidney Disease

We performed multiomics analysis; single nucleus RNA-seq (snRNA-seq) combined with ATAC (snATAC-seq) with 10XGenomics Multiome platform to generate cell-type-specific gene expression and chromatin accessibility atlas of the mouse polycystic kidney disease on a time course. Overall design: We inducedPkd1deletion in the tubular cells of Pkd1fl/fl; Pax8rtTA; TetO-Cremale mice from postnatal day 27 (P27) to 57 (P57) with doxycycline injection. The littermate male controls lacked either thePax8rtTA(P66) ortheTetOCremodule (P100, P130) but were treated with doxycycline in the same manner. The kidneys were harvested and snap-frozen at postnatal day 66 (P66), 100 (P100) or 130 (P130).