Monosaccharide profile of the extracts and the residues of extraction, from R.raetam young stems (YS) and adult stems (AS)

Prior to analysis, polysaccharidic fractions were submitted to methanolysis. The methylglucosides released obtained were then derivatized by trimethylsilylation. The analysis was carried out on 200 to 500 μg of freeze-dried polysaccharide powder, added to an internal standard, mesoinositol (MI), at a rate of 10% of the quantity of polysaccharide.
Methylglycosides trimethylsylilated have been identified by GLC by comparison with authentic samples using a Perichrom PR 2100 chromatograph equipped with a capillary tube (0.32 mm by 60 m) OPTIMA® 1-Accent 0.25 μM (Macherey-Nagel) and with a flame ionization detector (FID).
The chromatograph has been controlled by the software Winilab III (Perichrom). The results were expressed as a molar percentage after correction of the areas of the chromatographic peaks by the response factors.

在分析之前，多糖类组分经甲醇解处理。随后，所释放的甲基葡萄糖苷通过三甲基硅烷化进行衍生化。分析过程针对200至500微克的冷冻干燥多糖粉末进行，其中加入了内标物质中位醇（MI），其添加量为多糖量的10%。通过Perichrom PR 2100色谱仪（配备0.32毫米×60米的毛细管，OPTIMA® 1-Accent 0.25 μM，Macherey-Nagel），并配备火焰电离检测器（FID），对三甲基硅烷化甲基糖苷进行了鉴定，鉴定过程中与标准样品进行了比较。色谱仪由Winilab III（Perichrom）软件进行控制。结果以摩尔百分比表示，并经过色谱峰面积响应因子的校正。