In situ effect of a proanthocyanidin mouthrinse on dentin subjected to erosion

Abstract Proanthocyanidin has been shown to be efficient in inhibiting matrix metalloproteinases. Objective The aim of this in situ study was to evaluate the protective effect of Proanthocyanidin-based mouthrinses either with naturally acidic or with a neutral pH applied on dentin subjected to erosion. Methodology Eight volunteers wore one palatal device in two phases (7 days washout) with 16 samples per group (n=8). The groups under study were: First Phase/ G1 – 10% proanthocyanidin mouthrinse (pH 7.0, Experimental group 1 – Purified Grape Seeds Oligomeric Proanthocyanidins), G2 – 10% proanthocyanidin mouthrinse (pH 3.0, Experimental group 2 – Purified Grape Seeds Oligomeric Proanthocyanidins). Second Phase/ G3 – 0.12% chlorhexidine mouthrinse (pH 7.0, Positive control group), G4 – no previous treatment (Negative control group). Each device was subjected to 3 erosive cycles (5 minutes) per day for 5 days. Treatments with different mouthrinses were applied once after the second erosive challenge (5 minutes). Profilometry was used to quantify dentin loss (µm). Results Data were analyzed by repeated measures of ANOVA followed by Fisher’s test (p<0.05). G1 (1.17±0.69) and G3 (1.22±0.25) showed significantly lower wear values with no statistical difference between them. G2 (2.99±1.15) and G4 (2.29±1.13) presented higher wear values with no significant differences between them. Conclusion The 10% proanthocyanidin mouthrinse (pH 7.0) could be a good strategy to reduce dentin wear progression.

摘要 原花青素（Proanthocyanidin）已被证实可有效抑制基质金属蛋白酶（matrix metalloproteinases）。
研究目的 本原位研究旨在评估分别使用天然酸性及中性pH值的原花青素漱口水，对受侵蚀牙本质的保护作用。
研究方法 8名志愿者分两阶段佩戴腭部装置（洗脱期7天），每组设置16个样本（n=8）。受试分组如下：
第一阶段：G1——10%原花青素漱口水（pH 7.0，实验组1：纯化葡萄籽低聚原花青素（Purified Grape Seeds Oligomeric Proanthocyanidins））；G2——10%原花青素漱口水（pH 3.0，实验组2：纯化葡萄籽低聚原花青素（Purified Grape Seeds Oligomeric Proanthocyanidins））。
第二阶段：G3——0.12%氯己定（洗必泰，chlorhexidine）漱口水（pH 7.0，阳性对照组）；G4——未进行预处理（阴性对照组）。
每台装置每日进行3次侵蚀循环（每次5分钟），持续5天。在第二次侵蚀刺激（5分钟）后，单次使用对应漱口水进行处理。采用轮廓测厚仪定量测定牙本质流失量（单位：μm）。
研究结果 数据采用重复测量方差分析（repeated measures ANOVA）结合费希尔检验（Fisher’s test）进行统计学分析（p<0.05）。结果显示，G1组（1.17±0.69）与G3组（1.22±0.25）的磨耗值显著更低，且两组间无统计学差异；G2组（2.99±1.15）与G4组（2.29±1.13）磨耗值较高，两组间无显著差异。
研究结论 10%中性pH（pH 7.0）原花青素漱口水或可作为延缓牙本质磨耗进展的有效策略。