Additional file 1 of OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in Rice

Additional file 1: Fig. S1. Construction of OsGATA16 overexpression transgenic lines. (a) Schematic of recombinant overexpression plasmid with OsGATA16 under the control of a ubiquitin promoter. (b) Expression analysis of OsGATA16 in wild-type (WT) and overexpression (OE) lines by qRT-PCR. Data represent the mean ± SE from three replicates. Asterisks indicate significant differences in expression level (Student’s t-test, **p < 0.01). (c) Verification of transgenic OE lines by PCR. NC: negative control; M: 2000 bp marker. Fig. S2. Analysis of agronomic traits in wild-type (WT) and OsGATA16-overexpression (OE) lines under field conditions. (a) WT and OE plants at maturity. (b-c) Trait statistics for plant height (b) and hundred-grain weight (c) in WT and OE lines at maturity. Data represent the mean ± SE from three replicates. Fig. S3. Expression analysis of cold-sensitive genes in wild-type and OsGATA16-overexpression (OE) lines by qRT-PCR under normal and cold conditions. Data represent the mean ± SE from three replicates.

附加文件1：图S1。OsGATA16过表达转基因株系的构建。(a) 以泛素启动子（ubiquitin promoter）调控OsGATA16表达的重组过表达质粒示意图。(b) 通过实时荧光定量聚合酶链式反应（quantitative real-time PCR，qRT-PCR）分析野生型（WT）与过表达（OE）株系中OsGATA16的表达水平。数据以三次重复的平均值±标准误（mean ± SE）表示。星号代表表达水平存在显著差异（学生t检验（Student’s t-test），**p < 0.01）。(c) 通过聚合酶链式反应（PCR）验证转基因过表达株系。NC：阴性对照（negative control）；M：2000 bp分子量标记（marker）。
图S2。田间条件下野生型与OsGATA16过表达（OE）株系的农艺性状分析。(a) 成熟时期的野生型与过表达植株。(b-c) 成熟时期野生型与过表达株系的株高（b）与百粒重（c）性状统计。数据以三次重复的平均值±标准误（mean ± SE）表示。
图S3。正常与低温条件下，通过qRT-PCR分析野生型与OsGATA16过表达（OE）株系中冷敏感基因的表达水平。数据以三次重复的平均值±标准误（mean ± SE）表示。