Synthesis of new phenolic-containing hydroxyacyl probe and its colorimetric fluorescence detection of S^2-

The excessive S^2- can lead to a variety of serious physiological diseases, so the quickly detection of S^2- is of great significance for human. In this paper, a new simple and easily probe <b>Y</b> was designed and synthesized with hydrazine and substituted salicylaldehyde. It can detect S^2- with good performance in a highly water contain solution (DMSO/H₂O, v/<i>v</i> = 1/9), both colorimetric and fluorescence spectroscopy have obvious changes after adding 50-folds of S^2-, and other common fifteen anions have almost no influence in the detection. The detection limit and complexation constant were calculated using fluorescence titration data to be 3.88 μmol/L and 3.68×10⁶ L/mol, respectively. Job’s plot experiment showed that the binding ratio of the host with S^2- is <b>1:1</b>. The NMR titration was employed to verify the recognition mechanism, three protons in two hydroxyl groups (-OH) and one imine (-NH) are disappeared after adding excessive S^2-, which shown that probe <b>Y</b> undergo a deprotonation process. Preliminary application experiments show that probe <b>Y</b> can detect S^2- in water samples and soil.