Quantitative characterization of protein N-linked core-fucosylation by an efficient glycan truncation strategy

we developed a truncation strategy which efficiently convert intact glycopeptides with hundreds of different glycans into two truncated forms, i.e. a monosaccharide HexNAc and a disaccharide HexNAc+core-Fucose. Further combination with data-independent analysis to form an integrated platform allowed the measurement of site-specific core-fucosylation abundances and the determination of CF occupancy ratio with high reproducibility. Notably, three times of CF sites were identified using this strategy compared to conventional methods based on intact glycopeptide.