Genome wide methylation analysis of aged uterus and decidua

We use MeDIP-sequencing to measure global DNA methylation (5mC) in the uteri of young and aged C57BL/6 mice at day 3.5 (E3.5) of pregnancy, and of young and aged decidua at day 11.5 (E11.5) of pregnancy For E3.5 uterus MeDIP-seq data, we generated biological replicates from 3 young (8-20 week old) and 5 aged (41-49 week old) mice. For E11.5 data, we generated biological replicates of 6 deciduae from 3 litters (2 from each litter) from young mothers (9-10 weeks, n=3) and 10 deciduae from 5 litters of aged mothers (43-47 weeks, 2 deciduae from each litter). Aged E11.5 samples were split into 2 groups: those accompanying embryos that appeared normal (An, n=3) and those with morphologically abnormal embryos (Aa, n=3). One An and one Aa decidua was taken from each litter