Role of MyD88 intermediate domain in Myddosome complex assembly

The dataset provided contains the tables and figures for the MSc dissertation titled "The role of the MyD88 intermediate domain in the formation of the Myddosome assemble in innate immunity". This project set out to investigate the role of the MyD88 intermediate domain in the assembly of the Myddosome complex. Two variants of MyD88 were therefore expressed in addition to the IRAK-4 death domain to reconstitute the Myddosome complex. The two variants were the MyD88 death domain and MyD88 that contained both the death and intermediate domains. MyD88 that lacked the intermediate domain was found to be an oligomer using size exclusion chromatography and dynamic light scattering. However, MyD88 that contained only the death domain was monomeric.
MyD88 that contained both the death and intermediate domain was mixed with IRAK-4 death domain to reconstitute the Myddosome complex. As expected, we managed to form the Myddosome complex. The presence of this complex was confirmed by size exclusion chromatography and SDS-PAGE. On the contrary, the MyD88 death domain that lacked the intermediate domain failed to form the Myddosome complex when mixed with IRAK-4 death domain. This finding showed that the MyD88 intermediate domain was essential for the Myddosome assembly. It was also in support of existing literature that MyD88 does not interact with IRAK-4 in the absence of its intermediate domain.

所提供的数据集包含题为《MyD88中间域在固有免疫中Myddosome组装形成中的作用》的硕士论文中的表格和图表。该研究旨在探究MyD88中间域在Myddosome复合物组装中的作用。因此，除了IRAK-4死亡域外，还表达了两类MyD88变异体，以重建Myddosome复合物。这两类变异体分别是MyD88死亡域和同时包含死亡域和中间域的MyD88。缺乏中间域的MyD88通过尺寸排阻色谱法和动态光散射被证实为寡聚体。然而，仅包含死亡域的MyD88是单聚体。同时包含死亡域和中间域的MyD88与IRAK-4死亡域混合以重建Myddosome复合物。正如预期，我们成功地形成了Myddosome复合物。通过尺寸排阻色谱法和SDS-PAGE确认了该复合物的存在。相反，缺乏中间域的MyD88死亡域在与IRAK-4死亡域混合时未能形成Myddosome复合物。这一发现表明，MyD88中间域对于Myddosome组装是必不可少的。这也支持了现有文献，即在没有中间域的情况下，MyD88不与IRAK-4相互作用。