Additional file 3 of Comparison between 5 extractions methods in either plasma or serum to determine the optimal extraction and matrix combination for human metabolomics

Additional file 3: Figure S3. Quality analysis of plasmatic and serum extracts by SDS PAGE SDS-PAGE of loaded and eluted plasma and serum proteins, after five different deproteinizations. 1: Molecular Weight marker; 2: neat MeOH; 3: Plasma extract with MeOH; 4: neat MeOH:ACN; 5: Plasma extract MeOH:ACN; 6: neat ACN, 7: Plasma extract with ACN, 8: neat hybrid-SPE with ACN, 9: Plasma extract with hybrid-SPE with ACN; 10: neat hybrid-SPE with MeOH; 11: Plasma extract with hybrid-SPE with MeOH; 12: Molecular Weight marker; 13: neat MeOH; 14: Serum extract with MeOH; 15: neat MeOH:ACN; 16: Serum extract MeOH:ACN; 17: neat ACN, 18: Serum extract with ACN, 19: neat hybrid-SPE with ACN, 20: Serum extract with hybrid-SPE with ACN; 21: neat hybrid-SPE with MeOH; 22: Serum extract with hybrid-SPE with MeOH.

补充文件3：图S3。血浆与血清提取物的质量分析：针对经五种不同脱蛋白处理后的上样及洗脱血浆、血清蛋白，采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）进行分析。各泳道对应信息如下：1：分子量标志物；2：纯甲醇（MeOH）空白对照；3：甲醇法处理的血浆提取物；4：纯甲醇-乙腈混合液（MeOH:ACN）空白对照；5：甲醇-乙腈法处理的血浆提取物；6：纯乙腈（ACN）空白对照；7：乙腈法处理的血浆提取物；8：乙腈-混合模式固相萃取（hybrid-SPE）空白对照；9：乙腈-混合模式固相萃取法处理的血浆提取物；10：甲醇-混合模式固相萃取空白对照；11：甲醇-混合模式固相萃取法处理的血浆提取物；12：分子量标志物；13：纯甲醇（MeOH）空白对照；14：甲醇法处理的血清提取物；15：纯甲醇-乙腈混合液（MeOH:ACN）空白对照；16：甲醇-乙腈法处理的血清提取物；17：纯乙腈（ACN）空白对照；18：乙腈法处理的血清提取物；19：乙腈-混合模式固相萃取空白对照；20：乙腈-混合模式固相萃取法处理的血清提取物；21：甲醇-混合模式固相萃取空白对照；22：甲醇-混合模式固相萃取法处理的血清提取物。